Protein_Domain
Part:BBa_K351009:Design
Designed by: Dongchan Yang Group: iGEM10_KAIST-Korea (2010-10-25)
Front Part of modified FGFR
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 98
Illegal SpeI site found at 104
Illegal SpeI site found at 519
Illegal PstI site found at 84
Illegal PstI site found at 724 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 98
Illegal SpeI site found at 104
Illegal SpeI site found at 519
Illegal PstI site found at 84
Illegal PstI site found at 724
Illegal NotI site found at 90 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 98
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 98
Illegal SpeI site found at 104
Illegal SpeI site found at 519
Illegal PstI site found at 84
Illegal PstI site found at 724 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 98
Illegal SpeI site found at 104
Illegal SpeI site found at 519
Illegal PstI site found at 84
Illegal PstI site found at 724 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is the variable region of modified FGFR. So if you want to make receptor for other antigen should change this region.
Source
signal peptide is from yeast genome. first ig-like domain is from human basic fgfr sequence. single chain antibody sequence from 16A1 sequence.