Protein_Domain

Part:BBa_K351009:Design

Designed by: Dongchan Yang   Group: iGEM10_KAIST-Korea   (2010-10-25)


Front Part of modified FGFR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 98
    Illegal SpeI site found at 104
    Illegal SpeI site found at 519
    Illegal PstI site found at 84
    Illegal PstI site found at 724
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 98
    Illegal SpeI site found at 104
    Illegal SpeI site found at 519
    Illegal PstI site found at 84
    Illegal PstI site found at 724
    Illegal NotI site found at 90
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 98
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 98
    Illegal SpeI site found at 104
    Illegal SpeI site found at 519
    Illegal PstI site found at 84
    Illegal PstI site found at 724
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 98
    Illegal SpeI site found at 104
    Illegal SpeI site found at 519
    Illegal PstI site found at 84
    Illegal PstI site found at 724
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the variable region of modified FGFR. So if you want to make receptor for other antigen should change this region.


Source

signal peptide is from yeast genome. first ig-like domain is from human basic fgfr sequence. single chain antibody sequence from 16A1 sequence.

References